Alterations of L-type calcium current and cardiac function in CaMKII{delta} knockout mice.

ABSTRACT:

Rationale

Recent studies have highlighted important roles of CaMKII in regulating Ca(2+) handling and excitation-contraction coupling. However, the cardiac effect of chronic CaMKII inhibition has not been well understood.

Objective

We have tested the alterations of L-type calcium current (I(Ca)) and cardiac function in CaMKIIdelta knockout (KO) mouse left ventricle (LV).

Methods and results

We used the patch-clamp method to record I(Ca) in ventricular myocytes and found that in KO LV, basal I(Ca) was significantly increased without changing the transmural gradient of I(Ca) distribution. Substitution of Ba(2+) for Ca(2+) showed similar increase in I(Ba). There was no change in the voltage dependence of I(Ca) activation and inactivation. I(Ca) recovery from inactivation

SUBMITTER: Xu L 

PROVIDER: S-EPMC2923749 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature