Helicobacter pylori CagA protein polymorphisms and their lack of association with pathogenesis.

ABSTRACT:

Aim

To investigate Helicobacter pylori (H. pylori) CagA diversity and to evaluate the association between protein polymorphisms and the occurrence of gastric pathologies.

Methods

One hundred and twenty-two clinical isolates of H. pylori cultured from gastric biopsies obtained from Colombian patients with dyspepsia were included as study material. DNA extracted from isolates was used to determine cagA status, amplifying the C-terminal cagA gene region by polymerase chain reaction. One hundred and six strains with a single amplicon were sequenced and results were used to characterize the 3' variable region of the cagA gene. To establish the number and type of tyrosine phosphorylation motifs Glutamine acid-Proline-Isoleucine-Tyrosine-Alanine (EPIYA) bioinformatic analysis using

SUBMITTER: Acosta N 

PROVIDER: S-EPMC2923768 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature