Genome-wide DNA methylation analysis for diabetic nephropathy in type 1 diabetes mellitus.

ABSTRACT:

Background

Diabetic nephropathy is a serious complication of diabetes mellitus and is associated with considerable morbidity and high mortality. There is increasing evidence to suggest that dysregulation of the epigenome is involved in diabetic nephropathy. We assessed whether epigenetic modification of DNA methylation is associated with diabetic nephropathy in a case-control study of 192 Irish patients with type 1 diabetes mellitus (T1D). Cases had T1D and nephropathy whereas controls had T1D but no evidence of renal disease.

Methods

We performed DNA methylation profiling in bisulphite converted DNA from cases and controls using the recently developed Illumina Infinium HumanMethylation27 BeadChip, that enables the direct investigation of 27,578 individual cytosines at CpG l

SUBMITTER: Bell CG 

PROVIDER: S-EPMC2924253 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature