Systematic screening of Drosophila deficiency mutations for embryonic phenotypes and orphan receptor ligands.

ABSTRACT: This paper defines a collection of Drosophila deletion mutations (deficiencies) that can be systematically screened for embryonic phenotypes, orphan receptor ligands, and genes affecting protein localization. It reports the results of deficiency screens we have conducted that have revealed new axon guidance phenotypes in the central nervous system and neuromuscular system and permitted a quantitative assessment of the number of potential genes involved in regulating guidance of specific motor axon branches. Deficiency "kits" that cover the genome with a minimum number of lines have been established to facilitate gene mapping. These kits cannot be systematically analyzed for phenotypes, however, since embryos homozygous for many deficiencies in these kits fail to develop due to the loss of

SUBMITTER: Wright AP 

PROVIDER: S-EPMC2924397 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature