Macroautophagy and chaperone-mediated autophagy are required for hepatocyte resistance to oxidant stress.

ABSTRACT:

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The function of the lysosomal degradative pathway of autophagy in cellular injury is unclear, because findings in nonhepatic cells have implicated autophagy as both a mediator of cell death and as a survival response. Autophagic function is impaired in steatotic and aged hepatocytes, suggesting that in these settings hepatocellular injury may be altered by the decrease in autophagy. To delineate the specific function of autophagy in the hepatocyte injury response, the effects of menadione-induced oxidative stress were examined in the RALA255-10G rat hepatocyte line when macroautophagy was inhibited by a short hairpin RNA (shRNA)-mediated knockdown of the autophagy gene atg5. Loss of macroautophagy sensitized cells to apoptotic and necrotic death from normally nontoxic co

SUBMITTER: Wang Y 

PROVIDER: S-EPMC2924621 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature