Design and construction of a preparative-scale dynamic field gradient focusing apparatus.

ABSTRACT: A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, approximately O (10 mgs). This paper explains how the preparative-scale DFGF apparatus was designed and fabricated. Scaled-down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative-scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein.

SUBMITTER: Tracy NI 

PROVIDER: S-EPMC2925538 | biostudies-literature | 2008 Mar-Apr

REPOSITORIES: biostudies-literature