Contrasting chromatin organization of CpG islands and exons in the human genome.

ABSTRACT:

Background

CpG islands and nucleosome-free regions are both found in promoters. However, their association has never been studied. On the other hand, DNA methylation is absent in promoters but is enriched in gene bodies. Intragenic nucleosomes and their modifications have been recently associated with RNA splicing. Because the function of intragenic DNA methylation remains unclear, I explored the possibility of its involvement in splicing regulation.

Results

Here I show that CpG islands were associated not only with methylation-free promoters but also with nucleosome-free promoters. Nucleosome-free regions were observed only in promoters containing a CpG island. However, the DNA sequences of CpG islands predicted the opposite pattern, implying a limitation of sequence progra

SUBMITTER: Choi JK 

PROVIDER: S-EPMC2926781 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature