RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast.

ABSTRACT:

Background

The use of genome-wide RNA abundance profiling by microarrays and deep sequencing has spurred a revolution in our understanding of transcriptional control. However, changes in mRNA abundance reflect the combined effect of changes in RNA production, processing, and degradation, and thus, mRNA levels provide an occluded view of transcriptional regulation.

Results

To partially disentangle these issues, we carry out genome-wide RNA polymerase II (PolII) localization profiling in budding yeast in two different stress response time courses. While mRNA changes largely reflect changes in transcription, there remains a great deal of variation in mRNA levels that is not accounted for by changes in PolII abundance. We find that genes exhibiting 'excess' mRNA produced per Pol

SUBMITTER: Kim TS 

PROVIDER: S-EPMC2926786 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature