Identification of novel exons and transcribed regions by chimpanzee transcriptome sequencing.

ABSTRACT:

Background

We profile the chimpanzee transcriptome by using deep sequencing of cDNA from brain and liver, aiming to quantify expression of known genes and to identify novel transcribed regions.

Results

Using stringent criteria for transcription, we identify 12,843 expressed genes, with a majority being found in both tissues. We further identify 9,826 novel transcribed regions that are not overlapping with annotated exons, mRNAs or ESTs. Over 80% of the novel transcribed regions map within or in the vicinity of known genes, and by combining sequencing data with de novo splice predictions we predict several of the novel transcribed regions to be new exons or 3' UTRs. For approximately 350 novel transcribed regions, the corresponding DNA sequence is absent in the human referenc

SUBMITTER: Wetterbom A 

PROVIDER: S-EPMC2926789 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature