Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes.

ABSTRACT:

Objective

Osteoarthritic (OA) chondrocytes behave in an intrinsically deregulated manner, characterized by chronic loss of healthy cartilage and inappropriate differentiation to a hypertrophic-like state. IKKalpha and IKKbeta are essential kinases that activate NF-kappaB transcription factors, which in turn regulate cell differentiation and inflammation. This study was undertaken to investigate the differential roles of each IKK in chondrocyte differentiation and hypertrophy.

Methods

Expression of IKKalpha or IKKbeta was ablated in primary human chondrocytes by retro-transduction of specific short-hairpin RNAs. Micromass cultures designed to reproduce chondrogenesis with progression to the terminal hypertrophic stage were established, and anabolism and remodeling of the extr

SUBMITTER: Olivotto E 

PROVIDER: S-EPMC2927097 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature