Impact of RNA degradation on gene expression profiling.

ABSTRACT:

Background

Gene expression profiling is a highly sensitive technique which is used for profiling tumor samples for medical prognosis. RNA quality and degradation influence the analysis results of gene expression profiles. The impact of this influence on the profiles and its medical impact is not fully understood. As patient samples are very valuable for clinical studies, it is necessary to establish criteria for the RNA quality to be able to use these samples in later analysis.

Methods

To investigate the effects of RNA integrity on gene expression profiling, whole genome expression arrays were used. We used tumor biopsies from patients diagnosed with locally advanced rectal cancer. To simulate degradation, the isolated total RNA of all patients was subjected to heat-induced

SUBMITTER: Opitz L 

PROVIDER: S-EPMC2927474 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature