Entrapment neuropathy results in different microRNA expression patterns from denervation injury in rats.

ABSTRACT:

Background

To compare the microRNA (miRNA) expression profiles in neurons and innervated muscles after sciatic nerve entrapment using a non-constrictive silastic tube, subsequent surgical decompression, and denervation injury.

Methods

The experimental L4-L6 spinal segments, dorsal root ganglia (DRGs), and soleus muscles from each experimental group (sham control, denervation, entrapment, and decompression) were analyzed using an Agilent rat miRNA array to detect dysregulated miRNAs. In addition, muscle-specific miRNAs (miR-1, -133a, and -206) and selectively upregulated miRNAs were subsequently quantified using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR).

Results

In the soleus muscles, 37 of the 47 miRNAs (13.4% of the 350 unique miRNA

SUBMITTER: Rau CS 

PROVIDER: S-EPMC2927509 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature