Production of glycoprotein vaccines in Escherichia coli.

ABSTRACT:

Background

Conjugate vaccines in which polysaccharide antigens are covalently linked to carrier proteins belong to the most effective and safest vaccines against bacterial pathogens. State-of-the art production of conjugate vaccines using chemical methods is a laborious, multi-step process. In vivo enzymatic coupling using the general glycosylation pathway of Campylobacter jejuni in recombinant Escherichia coli has been suggested as a simpler method for producing conjugate vaccines. In this study we describe the in vivo biosynthesis of two novel conjugate vaccine candidates against Shigella dysenteriae type 1, an important bacterial pathogen causing severe gastro-intestinal disease states mainly in developing countries.

Results

Two different periplasmic carrier proteins, Acr

SUBMITTER: Ihssen J 

PROVIDER: S-EPMC2927510 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature