Bifunctional chimeric fusion proteins engineered for DNA delivery: optimization of the protein to DNA ratio.

ABSTRACT:

Background

Cell penetrating peptides (CPPs) have been used to deliver nucleotide-based therapeutics to cells, but this approach has produced mixed results. Ionic interactions and covalent bonds between the CPPs and the cargos may inhibit the effectiveness of the CPPs or interfere with the bioactivity of the cargos.

Methods

We have created a bifunctional chimeric protein that binds DNA using the p50 domain of the NF-kappaB transcription factor and is functionalized for delivery with the TAT CPP. The green fluorescent protein (GFP) has been incorporated for tracking delivery. The new chimeric protein, p50-GFP-TAT, was compared to p50-GFP, GFP-TAT and GFP as controls for the ability to transduce PC12 cells with and without oligonucleotide cargos.

Results

The p50-GFP-TAT

SUBMITTER: Gao S 

PROVIDER: S-EPMC2927806 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature