Inflammatory tendencies and overproduction of IL-17 in the colon of young NOD mice are counteracted with diet change.

ABSTRACT:

Objective

Dietary factors influence diabetes development in the NOD mouse. Diet affects the composition of microbiota in the distal intestine, which may subsequently influence intestinal immune homeostasis. However, the specific effects of antidiabetogenic diets on gut immunity and the explicit associations between intestinal immune disruption and type 1 diabetes onset remain unclear.

Research design and methods

Gut microbiota of NOD mice fed a conventional diet or ProSobee formula were compared using gas chromatography. Colonic lamina propria immune cells were characterized in terms of activation markers, cytokine mRNA and Th17 and Foxp3(+) T-cell numbers, using real-time PCR and flow cytometry. Activation of diabetogenic CD4 T-cells by purified B-cells was assessed in both

SUBMITTER: Alam C 

PROVIDER: S-EPMC2927946 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature