Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung.

ABSTRACT:

Background

Pancreatic cancer is still associated with devastating prognosis. Real progress in treatment options has still not been achieved. Therefore new models are urgently needed to investigate this deadly disease. As a part of this process we have established and characterized a new human pancreatic cancer cell line.

Methods

The newly established pancreatic cancer cell line PaCa 5061 was characterized for its morphology, growth rate, chromosomal analysis and mutational analysis of the K-ras, EGFR and p53 genes. Gene-amplification and RNA expression profiles were obtained using an Affymetrix microarray, and overexpression was validated by IHC analysis. Tumorigenicity and spontaneous metastasis formation of PaCa 5061 cells were analyzed in pfp-/-/rag2-/- mice. Sensitivity

SUBMITTER: Kalinina T 

PROVIDER: S-EPMC2927995 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature