Bisulfite Patch PCR enables multiplexed sequencing of promoter methylation across cancer samples.

ABSTRACT: Aberrant DNA methylation frequently occurs at gene promoters during cancer progression. It is important to identify these loci because they are often misregulated and drive tumorigenesis. Bisulfite sequencing is the most direct and highest resolution assay for identifying aberrant promoter methylation. Recently, genomic capture methods have been combined with next-generation sequencing to enable genome-scale surveys of methylation in individual samples. However, it is challenging to validate candidate loci identified by these approaches because an efficient method to bisulfite sequence more than 50 differentially methylated loci across a large number of samples does not exist. To address this problem, we developed Bisulfite Patch PCR, which enables highly multiplexed bisulfite PCR and sequ

SUBMITTER: Varley KE 

PROVIDER: S-EPMC2928506 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature