Differential gene expression in adipose stem cells cultured in allogeneic human serum versus fetal bovine serum.

ABSTRACT: In preclinical studies, human adipose stem cells (ASCs) have been shown to have therapeutic applicability, but standard expansion methods for clinical applications remain yet to be established. ASCs are typically expanded in the medium containing fetal bovine serum (FBS). However, sera and other animal-derived culture reagents stage safety issues in clinical therapy, including possible infections and severe immune reactions. By expanding ASCs in the medium containing human serum (HS), the problem can be eliminated. To define how allogeneic HS (alloHS) performs in ASC expansion compared to FBS, a comparative in vitro study in both serum supplements was performed. The choice of serum had a significant effect on ASCs. First, to reach cell proliferation levels comparable with 10% FBS, at least

SUBMITTER: Lindroos B 

PROVIDER: S-EPMC2928709 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature