A conserved stem loop motif in the 5'untranslated region regulates transforming growth factor-β(1) translation.

ABSTRACT: Transforming growth factor-β(1) (TGF-β(1)) regulates cellular proliferation, differentiation, migration, and survival. The human TGF-β(1) transcript is inherently poorly translated, and translational activation has been documented in relation to several stimuli. In this paper, we have sought to identify in cis regulatory elements within the TGF-β(1) 5'Untranslated Region (5'UTR). In silico analysis predicted formation of stable secondary structure in a G/C-rich element between nucleotides +77 to +106, and demonstrated that this element is highly conserved across species. Circular dichroism spectroscopy confirmed the presence of secondary structure in this region. The proximal 5'UTR was inhibitory to translation in reporter gene experiments, and mutation of the secondary structure motif inc

SUBMITTER: Jenkins RH 

PROVIDER: S-EPMC2928724 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature