Candesartan reduces the innate immune response to lipopolysaccharide in human monocytes.

ABSTRACT:

Objective

Inhibition of angiotensin II receptor type 1 (AT1) reduces chronic inflammation associated with hypertension. We asked whether AT1 receptor inhibition would reduce the innate inflammatory response induced by bacterial lipopolysaccharide (LPS).

Methods

We used unstimulated human circulating monocytes obtained from healthy donors by counterflow centrifugal elutriation. Monocytes were studied in vitro after incubation with LPS (50 ng/ml) with and without 1 mumol/l candesartan, an AT1 receptor blocker. Angiotensin II receptor mRNA expression was determined by reverse transcriptase-PCR and receptor binding by autoradiography; inflammatory factor mRNA expression was studied by reverse transcriptase-PCR and cytokine release by ELISA.

Results

Human monocytes did not

SUBMITTER: Larrayoz IM 

PROVIDER: S-EPMC2928995 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature