Gut microbial gene expression in mother-fed and formula-fed piglets.

ABSTRACT:

Background

Effects of diet on the structure and function of gut microbial communities in newborn infants are poorly understood. High-resolution molecular studies are needed to definitively ascertain whether gut microbial communities are distinct in milk-fed and formula-fed infants.

Methodology/principal findings

Pyrosequencing-based whole transcriptome shotgun sequencing (RNA-seq) was used to evaluate community wide gut microbial gene expression in 21 day old neonatal piglets fed either with sow's milk (mother fed, MF; n = 4) or with artificial formula (formula fed, FF; n = 4). Microbial DNA and RNA were harvested from cecal contents for each animal. cDNA libraries and 16S rDNA amplicons were sequenced on the Roche 454 GS-FLX Titanium system. Communities were similar at the

SUBMITTER: Poroyko V 

PROVIDER: S-EPMC2929194 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature