Genetic affinities within a large global collection of pathogenic Leptospira: implications for strain identification and molecular epidemiology.

ABSTRACT: Leptospirosis is an important zoonosis with widespread human health implications. The non-availability of accurate identification methods for the individualization of different Leptospira for outbreak investigations poses bountiful problems in the disease control arena. We harnessed fluorescent amplified fragment length polymorphism analysis (FAFLP) for Leptospira and investigated its utility in establishing genetic relationships among 271 isolates in the context of species level assignments of our global collection of isolates and strains obtained from a diverse array of hosts. In addition, this method was compared to an in-house multilocus sequence typing (MLST) method based on polymorphisms in three housekeeping genes, the rrs locus and two envelope proteins. Phylogenetic relationships

SUBMITTER: Nalam K 

PROVIDER: S-EPMC2929200 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature