Novel strong tissue specific promoter for gene expression in human germ cells.

ABSTRACT:

Background

Tissue specific promoters may be utilized for a variety of applications, including programmed gene expression in cell types, tissues and organs of interest, for developing different cell culture models or for use in gene therapy. We report a novel, tissue-specific promoter that was identified and engineered from the native upstream regulatory region of the human gene NDUFV1 containing an endogenous retroviral sequence.

Results

Among seven established human cell lines and five primary cultures, this modified NDUFV1 upstream sequence (mNUS) was active only in human undifferentiated germ-derived cells (lines Tera-1 and EP2102), where it demonstrated high promoter activity (approximately twice greater than that of the SV40 early promoter, and comparable to the routine

SUBMITTER: Kuzmin D 

PROVIDER: S-EPMC2929213 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature