Electrochemical surface plasmon resonance and waveguide-enhanced glucose biosensing with N-alkylaminated polypyrrole/glucose oxidase multilayers.

ABSTRACT: In this work, we report an electrochemical surface plasmon resonance/waveguide (EC-SPR/waveguide) glucose biosensor that could detect enzymatic reactions in a conducting polymer/glucose oxidase (GO(x)) multilayer thin film. In order to achieve a controlled enzyme electrode and waveguide mode, GO(x) (negatively charged) was immobilized with a water-soluble, conducting N-alkylaminated polypyrrole (positively charged) using the layer-by-layer (LbL) electrostatic self-assembly technique. The electrochemical and optical signals were simultaneously obtained from the composite LbL enzyme electrode upon the addition of glucose as mediated by the electroactivity and electrochromic property of the polypyrrole layers. Signal enhancement in EC-SPR detection is obtained by monitoring the doping-dedopin

SUBMITTER: Baba A 

PROVIDER: S-EPMC2929602 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature