Transgenic overexpression of Abcb11 enhances biliary bile salt outputs, but does not affect cholesterol cholelithogenesis in mice.

ABSTRACT:

Background

Cholesterol gallstone disease is a complex genetic trait and induced by multiple but as yet unknown genes. A major Lith gene, Lith1 was first identified on chromosome 2 in gallstone-susceptible C57L mice compared with resistant AKR mice. Abcb11, encoding the canalicular bile salt export pump in the hepatocyte, co-localizes with the Lith1 QTL region and its hepatic expression is significantly higher in C57L mice than in AKR mice.

Material and methods

To investigate whether Abcb11 influences cholesterol gallstone formation, we created an Abcb11 transgenic strain on the AKR genetic background and fed these mice with a lithogenic diet for 56 days.

Result

We excluded functionally relevant polymorphisms of the Abcb11 gene and its promoter region between C57L and

SUBMITTER: Wang HH 

PROVIDER: S-EPMC2929639 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature