Indian hedgehog regulates intestinal stem cell fate through epithelial-mesenchymal interactions during development.

ABSTRACT:

Background & aims

Intestinal stem cells (ISCs) are regulated by the mesenchymal environment via physical interaction and diffusible factors. We examined the role of Indian hedgehog (Ihh) in mesenchymal organization and the mechanisms by which perturbations in epithelial-mesenchymal interactions affect ISC fate.

Methods

We generated mice with intestinal epithelial-specific disruption of Ihh. Gross and microscopic anatomical changes were determined using histologic, immunohistochemical, and in situ hybridization analyses. Molecular mechanisms were elucidated by expression profiling and in vitro analyses.

Results

Deletion of intestinal epithelial Ihh disrupted the intestinal mesenchymal architecture, demonstrated by loss of the muscularis mucosae, deterioration of the ex

SUBMITTER: Kosinski C 

PROVIDER: S-EPMC2930094 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature