Gap junction protein Cx37 interacts with endothelial nitric oxide synthase in endothelial cells.

ABSTRACT:

Objective

The gap junction protein connexin37 (Cx37) plays an important role in cell-cell communication in the vasculature. A C1019T Cx37 gene polymorphism, encoding a P319S substitution in the regulatory C terminus of Cx37 (Cx37CT), correlates with arterial stenosis and myocardial infarction in humans. This study was designed to identify potential binding partners for Cx37CT and to determine whether the polymorphism modified this interaction.

Methods and results

Using a high-throughput phage display, we retrieved 2 binding motifs for Cx37CT: WHK ... [K,R]XP ... and FHK ... [K,R]XXP ... , the first being more common for Cx37CT-319P and the second more common for Cx37CT-319S. One of the peptides (WHRTPRLPPPVP) showed 77.7% homology with residues 843 to 854 of endothelial nitr

SUBMITTER: Pfenniger A 

PROVIDER: S-EPMC2930827 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature