Synergistic interactions between aminoflavone, paclitaxel and camptothecin in human breast cancer cells.

ABSTRACT:

Purpose

Aminoflavone is a unique DNA damaging agent currently undergoing phase I evaluation in a prodrug form (AFP464). In anticipation of combination regimens, interactions between aminoflavone and several anticancer drugs were investigated in MCF-7 breast cancer cells to determine whether synergistic cancer cell killing effects were observed.

Methods

Colony formation assays were performed to assess the effect of combining aminoflavone with a variety of anticancer drugs. Changes in initial uptake, retention or efflux of aminoflavone and the second agent were compared to the behavior of drugs alone. Key features required for aminoflavone activity in cell culture models were also explored, focusing on the obligatory induction of CYP1A1/1A2 and binding of reactive aminoflavone

SUBMITTER: Reinicke KE 

PROVIDER: S-EPMC2931270 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature