Combinatorial approach to determine functional group effects on lipidoid-mediated siRNA delivery.

ABSTRACT: The application of RNA interference (RNAi), either in the clinic or in the laboratory, requires safe and effective delivery methods. Here, we develop a combinatorial approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether, or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we studied the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in v

SUBMITTER: Mahon KP 

PROVIDER: S-EPMC2931596 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature