GPR55-dependent and -independent ion signalling in response to lysophosphatidylinositol in endothelial cells.

ABSTRACT:

Background and purpose

The glycerol-based lysophospholipid lysophosphatidylinositol (LPI) is an endogenous agonist of the G-protein-coupled receptor 55 (GPR55) exhibiting cannabinoid receptor-like properties in endothelial cells. To estimate the contribution of GPR55 to the physiological effects of LPI, the GPR55-dependent and -independent electrical responses in this cell type were investigated.

Experimental approach

Applying small interference RNA-mediated knock-down and transient overexpression, GPR55-dependent and -independent effects of LPI on cytosolic free Ca(2+) concentration, membrane potential and transmembrane ion currents were studied in EA.hy296 cells.

Key results

In a GPR55-dependent, GDPbetaS and U73122-sensitive manner, LPI induced rapid and transient

SUBMITTER: Bondarenko A 

PROVIDER: S-EPMC2931756 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature