Transcription of Alu DNA elements in blood cells of sporadic Creutzfeldt-Jakob disease (sCJD).

ABSTRACT: Alu DNA elements were long considered to be of no biological significance and thus have been only poorly defined. However, in the past Alu DNA elements with well-defined nucleotide sequences have been suspected to contribute to disease, but the role of Alu DNA element transcripts has rarely been investigated. For the first time, we determined in a real-time approach Alu DNA element transcription in buffy coat cells isolated from the blood of humans suffering from sporadic Creutzfeldt-Jakob disease (sCJD) and other neurodegenerative disorders. The reverse transcribed Alu transcripts were amplified and their cDNA sequences were aligned to genomic regions best fitted to database genomic Alu DNA element sequences deposited in the UCSC and NCBI data bases. Our cloned Alu RNA/cDNA sequences were

SUBMITTER: Kiesel P 

PROVIDER: S-EPMC2933056 | biostudies-literature | 2010 Apr-Jun

REPOSITORIES: biostudies-literature