Dedifferentiation and proliferation of mammalian cardiomyocytes.

ABSTRACT:

Background

It has long been thought that mammalian cardiomyocytes are terminally-differentiated and unable to proliferate. However, myocytes in more primitive animals such as zebrafish are able to dedifferentiate and proliferate to regenerate amputated cardiac muscle.

Methodology/principal findings

Here we test the hypothesis that mature mammalian cardiomyocytes retain substantial cellular plasticity, including the ability to dedifferentiate, proliferate, and acquire progenitor cell phenotypes. Two complementary methods were used: 1) cardiomyocyte purification from rat hearts, and 2) genetic fate mapping in cardiac explants from bi-transgenic mice. Cardiomyocytes isolated from rodent hearts were purified by multiple centrifugation and Percoll gradient separation steps, and t

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC2933247 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature