A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein.

ABSTRACT:

Background

The hemagglutinin-neuraminidase (HN) protein is the major antigenic determinant of the Mumps virus (MuV) and plays an important role in the viral infectious cycle through its hemagglutination/hemadsorption (HA/HD) and neuraminidase (NA) activities.

Objective

analyze the biological and immunological properties of a polypeptide derived from a highly conserved region of the HN ectodomain.

Methods

a highly conserved region of the HN gene among several MuV genotypes was chosen to be cloned in a eukaryotic expression vector. The pcDNAHN176-construct was transfected into Vero cells and RNA expression was detected by RT-PCR, while the corresponding polypeptide was detected by immunofluorescence and immunochemistry techniques. The HD and NA activities were also meas

SUBMITTER: Herrera E 

PROVIDER: S-EPMC2933592 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature