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Synthesis and evaluation of new spacers for use as dsDNA end-caps.

ABSTRACT: A series of aliphatic and aromatic spacer molecules designed to cap the ends of DNA duplexes have been synthesized. The spacers were converted into dimethoxytrityl-protected phosphoramidites as synthons for oligonucleotides synthesis. The effect of the spacers on the stability of short DNA duplexes was assessed by melting temperature studies. End-caps containing amide groups were found to be less stabilizing than the hexaethylene glycol spacer. End-caps containing either a terthiophene or a naphthalene tetracarboxylic acid diimide were found to be significantly more stabilizing. The former showed a preference for stacking above an A*T base pair. Spacers containing only methylene (-CH(2)-) and amide (-CONH-) groups interact weakly with DNA and consequently may be optimal for applications that require minimal influence on DNA structure but require a way to hold the ends of double-stranded DNA together.

SUBMITTER: Ng PS

PROVIDER: S-EPMC2933660 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Synthesis and evaluation of new spacers for use as dsDNA end-caps.

Ng Pei-Sze PS Laing Brian M BM Balasundarum Ganesan G Pingle Maneesh M Friedman Alan A Bergstrom Donald E DE

Bioconjugate chemistry 20100801 8

A series of aliphatic and aromatic spacer molecules designed to cap the ends of DNA duplexes have been synthesized. The spacers were converted into dimethoxytrityl-protected phosphoramidites as synthons for oligonucleotides synthesis. The effect of the spacers on the stability of short DNA duplexes was assessed by melting temperature studies. End-caps containing amide groups were found to be less stabilizing than the hexaethylene glycol spacer. End-caps containing either a terthiophene or a naph ...[more]

PMID: 20715857

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