Paracrine interaction between adipose-derived stromal cells and cranial suture-derived mesenchymal cells.

ABSTRACT:

Background

Adipose-derived stromal cells are a potential cell source for the successful healing of skeletal defects. In this study, the authors sought to investigate the potential for cranial suture-derived mesenchymal cells to promote the osteogenic differentiation of adipose-derived stromal cells. Various reports have previously examined the unique in vitro attributes of suture-derived mesenchymal cells; this study sought to extend those findings.

Methods

Suture-derived mesenchymal cells were isolated from wild-type mice (n = 30) from both fusing posterofrontal and patent sagittal sutures. Cells were placed in Transwell inserts with human adipose-derived stromal cells (n = 5 patients) with osteogenic differentiation medium with or without recombinant Noggin (10 to 400 ng/m

SUBMITTER: James AW 

PROVIDER: S-EPMC2933932 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature