Differential host response to LPS variants in amniochorion and the TLR4/MD-2 system in Macaca nemestrina.

ABSTRACT:

Objectives

Microbial-specific factors are likely critical in determining whether bacteria trigger preterm labor. Structural variations in lipopolysaccharide (LPS), a component of gram-negative bacteria, can determine whether LPS has an inflammatory (agonist) or anti-inflammatory (antagonist) effect through Toll-like receptor 4 (TLR4). Our objective was to determine whether amniochorion could discriminate between LPS variants in a nonhuman primate model. We also cloned Macaca nemestrina TLR4 and MD-2 and compared this complex functionally to the human homologue to establish whether nonhuman primates could be used to study TLR4 signaling in preterm birth.

Study design

Amniochorion explants from M. nemestrina were stimulated with a panel of LPS variants for 24 h. Supernatants w

SUBMITTER: Chang J 

PROVIDER: S-EPMC2934902 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature