Circadian rhythms in Neurospora crassa: dynamics of the clock component frequency visualized using a fluorescent reporter.

ABSTRACT: The frequency (frq) gene of Neurospora crassa has long been considered essential to the function of this organism's circadian rhythm. Increasingly, deciphering the coupling of core oscillator genes such as frq to the output pathways of the circadian rhythm has become a major focus of circadian research. To address this coupling it is critical to have a reporter of circadian activity that can deliver high resolution spatial and temporal information about the dynamics of core oscillatory proteins such as FRQ. However, due to the difficulty of studying the expression of circadian rhythm genes in aerobic N. crassa cultures, little is known about the dynamics of this gene under physiologically realistic conditions. To address these issues we report a fluorescent fusion to the frq gene using a c

SUBMITTER: Castro-Longoria E 

PROVIDER: S-EPMC2935182 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature