Improved peak capacity for CE separations of enzyme inhibitors with activity-based detection using magnetic bead microreactors.

ABSTRACT: A technique for separating and detecting enzyme inhibitors was developed using CE with an enzyme microreactor. The on-column enzyme microreactor was constructed using NdFeB magnet(s) to immobilize alkaline phosphatase-coated superparamagnetic beads (2.8 microm diameter) inside a capillary before the detection window. Enzyme inhibition assays were performed by injecting a plug of inhibitor into a capillary filled with the substrate, AttoPhos. Product generated in the enzyme microreactor was detected by LIF. Inhibitor zones electrophoresed through the capillary, passed through the enzyme microreactor, and were observed as negative peaks due to decreased product formation. The goal of this study was to improve peak capacities for inhibitor separations relative to previous studies, which combi

SUBMITTER: Yan X 

PROVIDER: S-EPMC2935249 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature