Behavior of a metabolic cycling population at the single cell level as visualized by fluorescent gene expression reporters.

ABSTRACT:

Background

During continuous growth in specific chemostat cultures, budding yeast undergo robust oscillations in oxygen consumption that are accompanied by highly periodic changes in transcript abundance of a majority of genes, in a phenomenon called the Yeast Metabolic Cycle (YMC). This study uses fluorescent reporters of genes specific to different YMC phases in order to visualize this phenomenon and understand the temporal regulation of gene expression at the level of individual cells within the cycling population.

Methodology

Fluorescent gene expression reporters for different phases of the YMC were constructed and stably integrated into the yeast genome. Subsequently, these reporter-expressing yeast were used to visualize YMC dynamics at the individual cell level in cul

SUBMITTER: Laxman S 

PROVIDER: S-EPMC2935372 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature