Mlck1a is expressed in zebrafish thrombocytes and is an essential component of thrombus formation.

ABSTRACT:

Background

We have used the advantages of the zebrafish model system to demonstrate which of the vertebrate myosin light chain kinase (MLCK) genes is expressed in thrombocytes and important for thrombus formation.

Methods and results

Here we report that Mlck1a is an essential component of thrombus formation. Phylogenetic data revealed four zebrafish orthologous for three human MLCK genes. To investigate expression of the zebrafish mlck genes in thrombocytes we compared GFP-tagged platelets with other cells by microarray analysis, and showed that mlck1a expression was 4.5-fold enriched in platelets. Furthermore, mlck1a mRNA and mRNA for the platelet-specific cd41 co-localized in thrombi. Expression of other mlck subtypes was lower in GFP-tagged platelets (mlck1b; 0.77-fold en

SUBMITTER: Tournoij E 

PROVIDER: S-EPMC2935642 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature