Modules for C-terminal epitope tagging of Tetrahymena genes.

ABSTRACT: Although epitope tagging has been widely used for analyzing protein function in many organisms, there are few genetic tools for epitope tagging in Tetrahymena. In this study, we describe several C-terminal epitope tagging modules that can be used to express tagged proteins in Tetrahymena cells by both plasmid- and PCR-based strategies.

SUBMITTER: Kataoka K 

PROVIDER: S-EPMC2935961 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature