Validation of a constraint-based model of Pichia pastoris metabolism under data scarcity.

ABSTRACT:

Background

Constraint-based models enable structured cellular representations in which intracellular kinetics are circumvented. These models, combined with experimental data, are useful analytical tools to estimate the state exhibited (the phenotype) by the cells at given pseudo-steady conditions.

Results

In this contribution, a simplified constraint-based stoichiometric model of the metabolism of the yeast Pichia pastoris, a workhorse for heterologous protein expression, is validated against several experimental available datasets. Firstly, maximum theoretical growth yields are calculated and compared to the experimental ones. Secondly, possibility theory is applied to quantify the consistency between model and measurements. Finally, the biomass growth rate is excluded from

SUBMITTER: Tortajada M 

PROVIDER: S-EPMC2936294 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature