Modulation of the apelin/APJ system in heart failure and atherosclerosis in man.

ABSTRACT:

Background and purpose

The aim of this study was to determine whether the apelin/APJ system is altered in human cardiovascular disease by investigating whether the expression of apelin or its receptor is altered at the protein level.

Experimental approach

Radioligand binding studies were used to determine apelin receptor density in human cardiac tissues. Apelin peptide levels in cardiovascular tissues were determined by radioimmunoassay. In vitro pharmacology was used to assess vasoactive properties of apelin in human coronary artery. Localization of apelin and its receptor in coronary artery was determined using immunohistochemistry.

Key results

Apelin receptor density was significantly decreased in left ventricle from patients with dilated cardiomyopathy or ischaemi

SUBMITTER: Pitkin SL 

PROVIDER: S-EPMC2936849 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature