Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae.

ABSTRACT: The vast landscape of RNA-protein interactions at the heart of post-transcriptional regulation remains largely unexplored. Indeed it is likely that, even in yeast, a substantial fraction of the regulatory RNA-binding proteins (RBPs) remain to be discovered. Systematic experimental methods can play a key role in discovering these RBPs--most of the known yeast RBPs lack RNA-binding domains that might enable this activity to be predicted. We describe here a proteome-wide approach to identify RNA-protein interactions based on in vitro binding of RNA samples to yeast protein microarrays that represent over 80% of the yeast proteome. We used this procedure to screen for novel RBPs and RNA-protein interactions. A complementary mass spectrometry technique also identified proteins that associate wi

SUBMITTER: Tsvetanova NG 

PROVIDER: S-EPMC2937035 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature