Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses.

ABSTRACT: The rapid and accurate identification of pathogens is critical in the control of infectious disease. To this end, we analyzed the capacity for viral detection and identification of a newly described high-density resequencing microarray (RMA), termed PathogenID, which was designed for multiple pathogen detection using database similarity searching. We focused on one of the largest and most diverse viral families described to date, the family Rhabdoviridae. We demonstrate that this approach has the potential to identify both known and related viruses for which precise sequence information is unavailable. In particular, we demonstrate that a strategy based on consensus sequence determination for analysis of RMA output data enabled successful detection of viruses exhibiting up to 26% nucleotid

SUBMITTER: Dacheux L 

PROVIDER: S-EPMC2937603 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature