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Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

ABSTRACT: We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

SUBMITTER: Hrncirova K 

PROVIDER: S-EPMC2937726 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

Hrncirova Kristyna K   Lengerova Martina M   Kocmanova Iva I   Racil Zdenek Z   Volfova Pavlina P   Palousova Dita D   Moulis Mojmir M   Weinbergerova Barbora B   Winterova Jana J   Toskova Martina M   Pospisilova Sarka S   Mayer Jiri J  

Journal of clinical microbiology 20100630 9

We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics. ...[more]

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