Toward a quantitative modeling of the synthesis of the pectate lyases, essential virulence factors in Dickeya dadantii.

ABSTRACT: A dynamic mathematical model has been developed and validated to describe the synthesis of pectate lyases (Pels), the major virulence factors in Dickeya dadantii. This work focuses on the simultaneous modeling of the metabolic degradation of pectin by Pel enzymes and the genetic regulation of pel genes by 2-keto-3-deoxygluconate (KDG), a catabolite product of pectin that inactivates KdgR, one of the main repressors of pel genes. This modeling scheme takes into account the fact that the system is composed of two time-varying compartments: the extracellular medium, where Pel enzymes cleave pectin into oligomers, and the bacterial cytoplasm where, after internalization, oligomers are converted to KDG. Using the quasi-stationary state approximations, the model consists of some nonlinear differ

SUBMITTER: Kepseu WD 

PROVIDER: S-EPMC2937882 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature