Evaluation of hydrodynamic limb vein injections in nonhuman primates.

ABSTRACT: The administration route is emerging as a critical aspect of nonviral and viral vector delivery to muscle, so as to enable gene therapy for disorders such as muscular dystrophy. Although direct intramuscular routes were used initially, intravascular routes are garnering interest because of their ability to target multiple muscles at once and to increase the efficiency of delivery and expression. For the delivery of naked plasmid DNA, our group has developed a hydrodynamic, limb vein procedure that entails placing a tourniquet over the proximal part of the target limb to block all blood flow and injecting the gene vector rapidly in a large volume so as to enable the gene vector to be extravasated and to access the myofibers. The present study was conducted in part to optimize the procedure

SUBMITTER: Hegge JO 

PROVIDER: S-EPMC2938361 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature