Lysophosphatidic Acid Upregulates Laminin-332 Expression during A431 Cell Colony Dispersal.

ABSTRACT: Lysophosphatidic acid (LPA) is a bioactive phospholipid that affects various biological functions, such as cell proliferation, migration, survival, wound healing, and tumor invasion through LPA receptors. Previously, we reported that LPA induces A431 colony dispersal, accompanied by disruption of cell-cell contacts and cell migration. However, it remains unclear how LPA affects cell migration and gene expression during A431 colony dispersal. In this paper, we performed cDNA microarray analysis to investigate this question by comparing gene expression between untreated and LPA-treated A431 cells. Interestingly, these results revealed that LPA treatment upregulates several TGF-β1 target genes, including laminin-332 (Ln-332) components (α3, β3, and γ2 chains). Western blot analysis also showe

SUBMITTER: Yamashita H 

PROVIDER: S-EPMC2938436 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature