High-resolution differential ion mobility separations using planar analyzers at elevated dispersion fields.

ABSTRACT: The ion mobility spectrometry (IMS) methods are grouped into conventional IMS, based on the absolute ion mobility, and differential or field asymmetric waveform IMS (FAIMS), based on mobility differences between strong and weak electric fields. A key attraction of FAIMS is substantial orthogonality to mass spectrometry (MS). Although several FAIMS/MS platforms were commercialized, their utility was limited by FAIMS resolving power, typically ∼10-20. Recently, gas mixtures comprising up to 75% He have enabled resolving power >100 that permits separation of numerous heretofore "coeluting" isomers. This performance opens major new proteomic and other biological applications. Here, we show that raising the separation field by ∼35% over the previous 21 kV/cm provides similar or better resolutio

SUBMITTER: Shvartsburg AA 

PROVIDER: S-EPMC2939208 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature